Novel Point Mutations of the ace-1 Gene of Aedes aegypti Larva Treated with Methanolic Extract of Citrus hystrix

Introduction: The mosquito species of Ae. aegypti is a vector arthropod-borne diseases such as dengue haemorrhagic fever. Acetylcholinesterase (AChE) enzyme in that encoded by the ace-1 gene. Damage gene target insecticide lead to loss normal structure and function AChE. However, damage remains uncharacterised. main aim this study was find out point mutations larvae treated with methanolic extract Citrus hystrix leaves. Method: This experiment using completely randomized design two treatment groups. A container containing lethal concentration 50 C. leaves, control group only water 0.5% Tween-20. Each contained third instar aegypti, each repeated four times. Observation performed for 24 h number survived dead larvae. Survived were collected prior DNA extraction, PCR, electrophoresis, sequencing. sequences those groups then compared determine genetyx ver 12. Results: PCR products both showed clear bands 500-600 bp long. Furthermore, presence mutation confirmed sequencing product between group. larva extract-treated more Conclusions: first report indicated many form deletions insertions nitrogenous bases different amino acid variations after leaves than